Chemical Investigations in Kelussia odoratissima Mozaff. Leaves Based on Comprehensive Analytical Methods: LC-MS, SPME, and GC-MS Analyses

Kelussia odoratissima Mozaff. is a species of Apiaceae endemic to the Zagros Mountains in Iran. In present investigation, for first time, polyphenolic compounds and flavonoids its leaves were determined by liquid chromatography-mass spectrometry (LC-MS). As result, p-coumaric acid, ferulic caffeic chlorogenic acetyl phloroglucinol, vanillic m-coumaric 4-methylsiringol as main phenolic compounds, while 3-hydroxyflavone, flavone, quercetin, rutin, neohesperidin, polydatin, diosmin flavonoid components, which acid (303.08 µL/gDW), neohesperidin (38.37 µL/gDw), (28.62 µL/gDW) most abundant. Solid-phase microextraction (SPME) was also used determine chemical compounds. Based on SPME, (Z)-undec-6-en-2-one (17.48%) (Z)-butylidenephthalide (4.348%) major components. GC-MS analyses, (Z)-ligustilide compound; however, some new determined, including 3-ethylisobenzofuran-1 (3H)-one, (E)-ligugustilide, E-n-butylidene phthalide. Also, we have identified EOs ethyl isobutyl phthalides basis obtained EI-MS spectra. Finally, fragmentation discussed this research.